沈锦波
沈锦波博士、教授、博导，国家级青年人才，省部共建亚热带森林培育国家重点实验室PI。分别于2001、2008年于浙江师范大学获得理学学士、硕士学位。2013年于香港中文大学生物系获得博士学位，之后在该单位从事科研工作，先后担任博士后(Post-Doc Fellowship)，副研究员(Research Associate)。2018年进入浙江农林大学，主要从事植物囊泡运输过程在种子营养物质贮藏、植物生长发育过程逆境响应的调控作用机理研究。结合细胞生物学、分子生物学、遗传学、蛋白质组学等技术方法，在农林作物、模式植物中重点研究了蛋白质、油脂的囊泡运输途径。重点关注液泡前体或多囊泡体介导的液泡蛋白从反面高尔基体网状结构（TGN）到液泡的转运途径；通过研究植物亚细胞水平的pH，分析内膜系统pH调控囊泡运输的机制。这些基础理论研究将有助于提高农林作物种子内液泡贮藏蛋白、油脂的含量和品质，提高农林作物逆境适应能力，将助力高营养价值种子的培育。
Jinbo Shen is a Professor in the State Key Laboratory of Subtropical Silviculture at Zhejiang A&F University. He completed his Ph.D. in the lab of Prof. Liwen Jiang at The Chinese University of Hong Kong. He continued in the same group as a postdoctoral researcher, before taking the current position in ZAFU in 2018. His current research mainly focuses on dissecting the molecular mechanisms of protein trafficking in the endosomal-vacuole system in plants, as well as their potential application in plant biotechnology and silviculture.
专业领域 (Areas of expertise)
Protein targeting, protein traffic and secretion, vacuole, organelle biogenesis/function, plant biotechnology.
发表论文 (Publications)
一作（或共一）、通讯 As the first (co-first author) & Corresponding author:
1. Jinbo Shen# and Rui Zhao. (2023) Live measurement of organelle pH by plant pHluorins. Trends in Plant Science. 28(4):486-487.
2. Jan Zouhar#, Wenhan Cao, Jinbo Shen#, Enrique Rojo#. (2023) Retrograde transport in plants: Circular economy in the endomembrane system. European Journal of Cell Biology 102: 151309.
3. Hu S, Li B, Wu F, Zhu D, Zouhar J, Gao C, Shimada T, Rojo E, Hara-Nishimura I, Jiang L, Jinbo Shen#. (2022) Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins. Proc Natl Acad Sci U S A.;119(20):e2200492119. doi: 10.1073/pnas.2200492119.
4. Zhang M*, Hu S*, Yi F, Gao Y, Zhu D, Wang Y, Cai Y, Hou D, Lin X and Jinbo Shen# (2021) Organelle Visualization With Multicolored Fluorescent Markers in Bamboo. Frontiers in Plant Science. 12:658836.
5. Zhang Xi*, Man Yi*, Zhuang Xiaohong*, Shen Jinbo*, Zhang Yi*, Cui Yaning, Yu Meng, Xing Jingjing, Wang Guangchao, Lian Na, Hu Zijian, Ma Lingyu, Shen Weiwei, Yang Shunyao, Xu Huimin, Bian Jiahui, Jing Yanping, Li Xiaojuan, Li Ruili, Mao Tonglin, Jiao Yuling, Sodmergen Sodmergen, Ren Haiyun and Lin Jinxing#. Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques. SCIENCE CHINA Life Sciences. 64(9):1392-1422.
6. Hu, S., Li, Y., Jinbo Shen# (2020) A Diverse Membrane Interaction Network for Plant Multivesicular Bodies: Roles in Proteins Vacuolar Delivery and Unconventional Secretion. Frontiers in Plant Science. 11: 425. doi: 10.3389/fpls.2020.00425
7. Zhao, Q. *#, Jinbo Shen*, Gao, C.J.*, Cui, Y.*, Wang, Y.Y., Cui, J., Cheng, L.X., Cao, W.H., Zhu, Y., Huang, S.X., Zhou, Q.Z., Leong, C.K., Leung, P., Chen, X.M. and Jiang, L.W#. (2019) RST1 Is a FREE1 Suppressor That Negatively Regulates Vacuolar Trafficking in Arabidopsis. Plant Cell 31, 2152-2168.
8. 崔勇*#, 庄小红*#, 沈锦波*#, 高彩吉*#, 姜里文#(2019) 植物内膜系统细胞器生物发生及功能的分子机制研究. 中国科学: 生命科学 49, 1679; doi: 10.1360/SSV-2019-0136
9. Zhu, D., Zhang, M., Gao, C., and Jinbo Shen# (2020) Protein trafficking in plant cells: tools and markers. SCIENCE CHINA Life Sciences 63, 343.
10. Jinbo Shen*#, Qiong Zhao*, Xiangfeng Wang, Caiji Gao, Ying Zhu, Yonglun Zeng, and Liwen Jiang# (2018) A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis. Nature Communications 9:3784. (#corresponding author)
11. Zeng Yonglun*, Jinbo Shen*, Li BY*, Jiang LW# (2018) Hormone modulates protein dynamics to regulate plant growth. Proc. Natl. Acad. Sci. USA 115: 3521-3523 (co-first)
12. Caiji Gao*, Xiaohong Zhuang*, Jinbo Shen*, Liwen Jiang# (2017) Plant ESCRT Complexes: Moving Beyond Endosomal Sorting. Trends in Plant Science. 22(11):986–998. (co-first)
13. Jinbo Shen*, Caiji Gao*, Qiong Zhao, Youshun Lin, Xiangfeng Wang, Xiaohong Zhuang, and Liwen Jiang# (2016) AtBRO1 functions in ESCRT-I complex to regulate multivesicular body protein sorting. Molecular Plant. 9(5): 760-763.
14. Yong Cui*, Jinbo Shen*, Caiji Gao, Xiaohong Zhuang, Junqi Wang, Liwen Jiang# (2016) Biogenesis of Plant Prevacuolar Multivesicular Bodies. Molecular Plant. 9(6): 774–786 (Cover; co-first)
15. Jinbo Shen, Yu Ding, Caiji Gao, Enrique Rojo and Liwen Jiang# (2014) N-linked glycosylation of AtVSR1 is important for vacuolar protein sorting in Arabidopsis. The Plant Journal, 80, 977-992.
16. Jinbo Shen, Pui Kit Suen, Xiangfeng Wang, Youshun Lin, Sze Wan Lo, Enrique Rojo, and Liwen Jiang# (2013) An in vivo expression system for the identification of cargo proteins of vacuolar sorting receptors in Arabidopsis culture cells. The Plant Journal. 75(6), 1003–1017.
17. Jinbo Shen, Yonglun Zeng, Xiaohong Zhuang, Lei Sun, Xiaoqiang Yao, Peter Pimpl, and Liwen Jiang# (2013) Organelle pH in the Arabidopsis Endomembrane System. Molecular Plant. 6 (5): 1419–1437. (封面论文; ESI 高被引论文; Highlighted in National Science Review “The first organelle-specific pH map of the endomembrane system of Arabidopsis cells. 2013. 00: 1-2. doi: 10.1093/nsr/nwt018”)
18. Jinbo Shen, Jiaxin Fu, Jin Ma, Xiangfeng Wang, Caiji Gao, ChuXiong Zhuang, Jianmin Wan, and Liwen Jiang# (2014) Isolation, Culture, and Transient Transformation of Plant Protoplasts. Current Protocols in Cell Biology. 63:2.8.1-2.8.17. (Invited Paper).
19. Hongfei Lu*#, Jinbo Shen*, Xiaoqian Jin, David B. Hannaway, Christopher Daly, Michael D. Halbleib. (2008) Determining Optimal Seeding Times for Tall Fescue Using Germination Studies and Spatial Climate Analysis. Agricultural and Forest Meteorology, 148: 931-941. (co-first)
20. Jinbo Shen, L. Y. Xu, X. Q. Jin, J. H. Chen and H. F. Lu#. (2008) Effect of temperature regime on germination of seed of perennial ryegrass (Lolium perenne). Grass and Forage Science, 63(2): 249-253.
21. Jinbo Shen, Lu Hong-Fei#, Peng Qiu-Fa, Zheng Ju-Fang, Tian Yu-Mei. (2008) FTIR Spectra of Sect. Oleifera, Sect. Paracamellia, and Sect. Camellia (Theaceae) with Reference to Their Taxonomic Significance. Journal of Systematics and Evolution, 46(2): 194-204. (Cover)
22. Jinbo Shen, Xia Li, Dandan Wang and Hongfei Lu#. (2007) In Vitro Culture of Crofton Weed: Considerable Potential for Fast and Convenient Plantlet Production. Weed Technology, 21: 445-452. IF 5-year =1.14
23. Hong-Fei Lu*#, Jinbo Shen*, Xiu-Yan Lin & Jia-Li Fu. (2008) Relevance of Fourier transform infrared spectroscopy and leaf anatomy for species classification in Camellia (Theaceae). Taxon, 57: 1274-1288.
24. Hongfei Lu*#, Jinbo Shen*, Weiguo Sang, Xinyi Zhang, Jinxing Lin. (2008) Pollen Viability, Pollination, Seed Set and Seed Germination of Crofton Weed (Eupatorium adenophorum) in China. Weed Science, 56: 42-51.
合作发表代表性论文Representative publications as the co-author
25. Li H, Huang R, Liao Y, Yang S, Feng B, Qin H, Zhou J, Zeng Y, Shen J, Zhuang X, Jiang L, Otegui MS, Zhang S, Gao C. (2023). plant-unique protein BLISTER coordinates with core retromer to modulate endosomal sorting of plasma membrane and vacuolar proteins. Proc Natl Acad Sci U S A. 120(1):e2211258120.
26. Zeng Y, Li B, Huang S, Li H, Cao W, Chen Y, Liu G, Li Z, Yang C, Feng L, Gao J, Lo SW, Zhao J, Shen Jinbo, Guo Y, Gao C, Dagdas Y, Jiang L. (2023) The plant unique ESCRT component FREE1 regulates autophagosome closure. Nat Communications. 14(1):1768.
27. Lin, Y., Zeng, Y., Zhu, Y., Shen, J., Ye, H., and Jiang, L. (2021). Plant Rho GTPase signaling promotes autophagy. Mol. Plant 14, 905-920.
28. Zhang L, Ma J, Liu H, Yi Q, Wang Y, Xing J, Zhang P, Ji S, Li M, Li J, Shen J, Lin J. (2021) SNARE proteins VAMP721 and VAMP722 mediate the post-Golgi trafficking required for auxin-mediated development in Arabidopsis. Plant J.108(2):426-440.
29. Zeng Y, Li B, Ji C, Feng L, Niu F, Deng C, Chen S, Lin Y, Cheung KCP, Shen J, Wong KB, Jiang L. (2021) A unique AtSar1D-AtRabD2a nexus modulates autophagosome biogenesis in Arabidopsis thaliana. Proc Natl Acad Sci U S A.118(17):e2021293118.
30. Delgadillo MO, Ruano G, Zouhar J, Sauer M, Shen J, Lazarova A, Sanmartín M, Lai LTF, Deng C, Wang P, Hussey PJ, Sánchez-Serrano JJ, Jiang L, Rojo E. MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway. Proc Natl Acad Sci U S A. 2020 May 5;117(18):9884-9895.
31. Ying Zhua, Changyang Jia, Wenhan Cao, Jinbo Shen, Qiong Zhao, Liwen Jiang. (2020) Identification and characterization of unconventional membrane protein trafficking regulators in Arabidopsis: A genetic approach. Journal of Plant Physiology 252, 153229.
32. Zhu, Y., Ji, C., Cao, W., Shen, J., Zhao, Q., and Jiang, L. (2020). Identification and characterization of unconventional membrane protein trafficking regulators in Arabidopsis: A genetic approach. J. Plant Physiol. 252, 153229.
33. Xiaojie Zhu, Ting Pan, Xiao Zhang, Ligang Fan, Francisco J. Quintero, Hong Zhao, Xiaomeng Su, Xiaojiao Li, Irene Villalta, Imelda Mendoza, Jinbo Shen, Liwen Jiang, Jose M. Pardo, Quan-Sheng Qiu# (2018) K+ efflux antiporters 4, 5 and 6 mediate pH and K+ homeostasis in endomembrane compartments. Plant Physiology, DOI: https://doi.org/10.1104/pp.18.01053
34. Kwon, Y., Shen, J., Lee, M.H., Geem, K.R., Jiang, L., and Hwang, I. # (2018). AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking. PNAS, 10.1073/pnas.1717204115
35. Chen Chen*, Hao Chen*, You-Shun Lin*, Jin-Bo Shen, Jun-Xiang Shan, Peng Qi, Min Shi, Mei-Zhen Zhu, Xue-Hui Huang, Qi Feng, Bin Han, Liwen Jiang, Ji-Ping Gao and Hong-Xuan Lin# (2014) A two-locus interaction causes interspecific hybrid weakness in rice. Nature Communications, 5, 3357.
36. Peng Qi*, Youshun Lin*, Xianjun Song*, Jinbo Shen, Wei Huang, Junxiang Shan, Mei-Zhen Zhu, Liwen Jiang, Jiping Gao and Hongxuan Lin# (2012) The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3. Cell Research. 22:1666–1680.
37. Junqi Wang, Jinbo Shen, Yi Cai, David G. Robinson, and Liwen Jiang# (2013) Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues. Journal of Experimental Botany. 64(6): 1587-1601.
38. Pui Kit Suen, Jinbo Shen, Samuel S.M. Sun, Liwen Jiang# (2010) Expression and characterization of two functional vacuolar sorting receptor (VSR) proteins, BP-80 and AtVSR4 from culture media of transgenic tobacco BY-2 cells. Plant Science. 179: 68-76.
39. Lin Y, Ding Y, Wang J, Shen Jinbo, Kung CH, Zhuang X, Cui Y, Yin Z, Xia Y, Lin H, Robinson DG1, Jiang L# (2015) Exocyst-Positive Organelles and Autophagosomes Are Distinct Organelles in Plants. Plant Physiology, 169(3):1917-32.
40. Caiji Gao, Xiaohong Zhuang, Yong Cui, Xi Fu, Yilin He, Qiong Zhao, Yonglun Zeng, Jinbo Shen, Ming Luo, and Liwen Jiang# (2015) Dual roles of an Arabidopsis ESCRT component FREE1 in regulating vacuolar protein transport and autophagic degradation. Proceedings of the National Academy of Sciences of the United States of America, 112, 1886-1891.
41. Fang Luo, Yu Hang Fong, Yonglun Zeng, Jinbo Shen, Liwen Jiang and Kam-Bo Wong# (2014) How Vacuolar Sorting Receptor Proteins Interact with Their Cargo Proteins: Crystal Structures of Apo and Cargo-Bound Forms of the Protease-Associated Domain from an Arabidopsis Vacuolar Sorting Receptor. The Plant Cell, 26, 3693-3708.
42. Qiong Zhao, Caiji Gao, PoShing Lee, Lin Liu, Shaofang Li, Tangjin Hu, Jinbo Shen, Shuying Pan, Hao Ye, Yunru Chen, Wenhan Cao, Yong Cui, Peng Zeng, Sheng Yu, Yangbin Gao, Liang Chen, Beixin Mo, Xin Liu, Shi Xiao, Yunde Zhao, Silin Zhong, Xuemei Chen, Liwen Jiang (2015) Fast-suppressor screening for new components in protein trafficking, organelle biogenesis and silencing pathway in Arabidopsis thaliana using DEX-inducible FREE1-RNAi plants. Journal of Genetics and Genomics. 42: 319-30.
43. Yansong Miao, Hong-Ye Li, Jinbo Shen, Junqi Wang, and Liwen Jiang (2011) QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells. Journal of Experimental Botany. 62(14): 5063-5078.
44. Bo Jiang, Zonggen Shen, Jinbo Shen, Yu Da, Xianyong Sheng, Hongfei Lu (2009) Germination and growth of sponge gourd (Luffa cylindrica) pollen tubes and FTIR analysis of the pollen tube wall. Scientia Horticulturae, 122, 638-644.
45. Hongfei Lu, Bo Jiang, Zonggen Shen, Jinbo Shen, Qiufa Peng, Cungui Cheng. (2008) Comparative leaf anatomy, FTIR discrimination and biogeographical analysis of the section Tuberculata (Camellia, Theaceae) with a discussion of its taxonomatic treatments. Plant Systematics and Evolution, 274: 223-235.
专著章节 (Book Chapter)
1. Jinbo Shen*, Xiangfeng Wang*, and Liwen Jiang. Seeds as bioreactors. Allison Kermode (Editor) Molecular Pharming: Applications, Challenges and Emerging Areas. 2018. ISBN: 978-1-118-80128-4.
2. Jinbo Shen: A Secretion System for Cargo Protein Identification of Vacuolar Sorting Receptors. Plant Protein Secretion, 09/2017: pages 183-191; ISBN: 978-1-4939-7261-6, DOI:10.1007/978-1-4939-7262-3_16
3. Jinbo Shen: The Organelle pH Estimate and Measurement in Plant Secretory Pathway. Plant Protein Secretion, 09/2017: pages 223-230; ISBN: 978-1-4939-7261-6, DOI:10.1007/978-1-4939-7262-3_20
4. Wenjin Shen, Zhidan Xiao, Jinbo Shen, Caiji Gao: Analysis of Golgi-Mediated Protein Traffic in Plant Cells. Plant Protein Secretion, 09/2017: pages 75-86; ISBN: 978-1-4939-7261-6, DOI:10.1007/978-1-4939-7262-3_6
5. Angus Ho Yin Law*, Jinbo Shen*, Liwen Jiang: SCAMP, VSR, and plant endocytosis. Endocytosis in Plants, Edited by Jozef Šamaj, 08/2012: pages 217-231; Springer Berlin Heidelberg., DOI:10.1007/978-3-642-32463-5_11
6. D.B. Hannaway, Christopher Daly, M.D. Halbleib, Daniel James, C.P. West, Jeffrey J. Volenec, David Chapman, Xianglin Li, Weixing Cao, Jinbo Shen, Xuezheng Shi, Steve Johnson: Development of Suitability Maps with Examples for the United States and China. Tall Fescue for the 21st Century, Edited by H.A. Fribourg, D.B. Hannaway, C.P. West, 01/2009: pages 33-47; ASA, CSSA, and SSSA Monograph., DOI:10.2134/agronmonogr53.c3
学术荣誉和奖励 (Honors and Awards)
国家青年人才. 2022；
浙江省高校领军人才培养计划高层次拔尖人才. 2020；
浙江省海外高层次人才-创新长期项目. 2017 (Talents Program Award of Zhejiang Province, Zhejiang Province Government, China, 2017)；
教育部自然科学二等奖. 2022 (MoE Natural Science Award of China, China Ministry of Education, 2nd Class, 2022)；
教育部自然科学二等奖. 2018 (MoE Natural Science Award of China, China Ministry of Education, 2nd Class, 2018)；
The Postgraduate Research Output Award of CUHK. 2014
Cold Spring Harbor Asia Fellowship Reward. 2011
学术兼职 (Professional Activities)
Faculty Opinion (F1000 Prime), Associate Faculty Member. 2021
Plant Cell, Chinese Language Assistant Features Editor. 2018
Frontiers in Plant Science, Review Editor. 2020
浙江省生化学会理事
中国电子显微镜学会第十一届共聚焦专业委员会委员.
本实验室长期招聘硕士、博士、博士后等研究人员！
For research requirements, Jinbo Shen’s group is recruiting domestic and international postdoctoral fellows and doctoral students. Talents from all over the world are welcome to join us!
通讯地址: 浙江省杭州市临安区武肃街666号，浙江农林大学国重大楼413室
Corresponding Address: Room 413, State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University (ZAFU), No.666 Wusu Street, Lin'an District, Hangzhou, Zhejiang, China.
E-mail: jshen#zafu.edu.cn（请将#换成@）
Web: https://www.researchgate.net/profile/Jinbo_SHEN
